Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fredrik Lindeberg
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-18 11:30:43
ModLac laccase with His-tag and HlyA export tag (inc RBS and J23110 promoter)
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1688007_sequence (Version 1) |
Description
Assembly of Anderson promoter BBa_J23110 and RBS + ModLac with HlyA tag (BBa_K1688006). ModLac is a modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus.Notes
The modified CueO was synthesized with iGEM prefix and suffix, RBS(BBa_B0034) and a M-scar after the RBS in order to guarantee the function of the RBS. The gene is followed by a KpnI restriction site with glycines and serines on each side, which are small amino acids which increase the flexibility of the protein. This is needed for the export tag HlyA , to make it easier for the resulting signal protein to be folded properly. A second KpnI restriction site is put after the HlyA so that the export tag can be removed easily. It has then two stop codons, and a terminator at the end.Source
D439A/M510L CueO mutant sequence received from Professor Kunishige Kataoka and synthesized with His-tag and HlyA export tag.| Access | Instance | Definition |
| public public | BBa_J23110 BBa_K1688006 | BBa_J23110 BBa_K1688006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23110 BBa_K1688006 | 1,35 44,1843 | BBa_J23110 BBa_K1688006 |