Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fredrik Lindeberg
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-18 11:30:53
ModLac laccase with His-tag (inc RBS and J23110 promoter)
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1688008_sequence (Version 1) |
Description
ModLac is a modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence.Notes
The gene is followed by a KpnI restriction site with glycines and serines on each side, which are small amino acids which increase the flexibility of the protein. The gene was originally synthesized with a C-terminus export tag attached. This tag was removed through digestion with Kpn1 restriction enzyme and resulted in this sequence.Source
D439A/M510L CueO mutant sequence received from Professor Kunishige Kataoka and synthesized with His-tag.| Access | Instance | Definition |
| public public public | BBa_J23110 BBa_B0034 BBa_K1688021 | BBa_J23110 BBa_B0034 ModLac |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23110 BBa_B0034 BBa_K1688021 | 1,35 44,55 62,1651 | BBa_J23110 BBa_B0034 ModLac |