BBa_K1689011

BBa_K1689011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1689011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiaofeng Li
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-27 10:05:18

dCas9-delta alpha



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1689011_sequence (Version 1)

Description

delta alpha segment of lacz fused with dCas9, His tag allows for protein purification

Notes

goldengate

Source

E.coli

Sequence Annotation Location Component / Role(s)
dCas9-delta alpha
His tag
GGGGS*2 Linker
dCas9
delta alpha
random spacer
start
stop
random spacer
34,7164
37,54
4156,4185
55,4155
4186,7161
1,33
34,37
7162,7164
7165,7235
CDS feature/cds
feature/tag tag
CDS feature/protein
feature/protein CDS
feature/protein CDS
feature/misc sequence_feature
start_codon feature/start
stop_codon feature/stop
sequence_feature feature/misc
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1689011/1