BBa_K1689012

BBa_K1689012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1689012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiaofeng Li
Date created: 2015-09-15 11:00:00
Date modified: 2015-11-17 09:39:04

dCas9-Nlact



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1689012_sequence (Version 1)

Description

NFluc of lactamase fused with dCas9, His tag allows for protein purification

Notes

goldengate

Source

E.coli

Sequence Annotation Location Component / Role(s)
His
dCas9-Nlact
dCas9
GGGGS*2 Linker
Nlact for N terminal of β-Lactamase
start
random spacer
stop
random spacer
37,54
34,4701
55,4155
4156,4185
4186,4698
34,36
1,33
4699,4701
4702,4772
tag feature/tag
feature/cds CDS
CDS feature/protein
feature/protein CDS
feature/protein CDS
feature/start start_codon
sequence_feature feature/misc
feature/stop stop_codon
feature/misc sequence_feature
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1689012/1