BBa_K1689013

BBa_K1689013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1689013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiaofeng Li
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-27 01:29:15

Nlact-dCas9



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1689013_sequence (Version 1)

Description

NFluc of lactamase fused with dCas9, His tag allows for protein purification

Notes

goldengate

Source

E.coli

Sequence Annotation Location Component / Role(s)
Nlact-dCas9
His
Nlact for N terminal of β-Lactamase
GGGGS*2 Linker
dCas9
start
random spacer
random spacer
stop
34,4701
37,54
55,567
568,697
598,4698
34,36
1,33
4701,4772
4699,4701
feature/cds CDS
tag feature/tag
feature/protein CDS
CDS feature/protein
CDS feature/protein
feature/start start_codon
sequence_feature feature/misc
feature/misc sequence_feature
feature/stop stop_codon
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1689013/1