BBa_K1689015

BBa_K1689015 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1689015
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei weijia
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-27 01:33:33

F[1,2]-dCas9



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1689015_sequence (Version 1)

Description

pSB1C3-6His-F[1,2]-dCas9, segment F[1,2] fusion with dCas9, 6His tag allows for protein purification.

Notes

the GC% is high of F[1,2]

Source

E.coli

Sequence Annotation Location Component / Role(s)
His
F[1,2]
dCas9
GGGGS*2 Linker
F[1,2]-dCas9
random spacer
start
stop
random spacer
37,54
55,315
346,4449
316,345
34,4452
1,33
34,36
4450,4452
4453,4520
feature/tag tag
feature/protein CDS
CDS feature/protein
CDS feature/protein
feature/cds CDS
sequence_feature feature/misc
feature/start start_codon
feature/stop stop_codon
feature/misc sequence_feature
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1689015/1