Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei weijia
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-27 01:38:42
F3-dCas9
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1689017_sequence (Version 1) |
Description
pSB1C3-6H-F3-dCas9, fusion protein of F3 segment of DHFR fused with dCas9, the His tag facilitates protein purificationNotes
The coding sequence of this fusion protein is too long, you'd better amplify is separately.Source
E.coli| Sequence Annotation | Location | Component / Role(s) |
| F3-dCas9 His tag F3 GGGGS*2 Linker dCas9 random spacer start stop random spacer | 34,4401 37,54 55,267 268,297 298,4398 1,33 34,36 4398,4401 4402,4472 | feature/cds CDS feature/tag tag CDS feature/protein CDS feature/protein CDS feature/protein sequence_feature feature/misc feature/start start_codon stop_codon feature/stop feature/misc sequence_feature |