BBa_K1690001

BBa_K1690001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1690001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anna Sosnova, Pavel Zach

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-17 05:33:58

Mating pheromone (C. albicans)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1690001_sequence (Version 1)

Description

This is a coding sequence of C. albicans mating pheromone together with a secretion tag. Expression in the yeast cell causes secretion of the mature mating pheromone into the medium. Cells with a corresponding STE2 receptor react to this pheromone by the activation of the pheromone mating pathway. Detailed description can be obtained from the iGEM 2015 Czech Republic wiki.

Notes

This part is derived from BBa_K1690000. Using site-directed mutagenesis, we replaced the original pheromone sequence.

Source

The secretion tag was obtained by a genomic PCR from S. cerevisiae. The pheromone sequence was synthetized.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Kozak	1,6	feature/rbs, ribosome_entry_site
start	7,9	feature/start, start_codon
Secretion tag	10,275	tag, feature/tag
Pheromone	276,312	feature/dna, sequence_feature
stop	313,315	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1690001/1

Member of these Collections

• iGEM Parts Registry