BBa_K1690003

BBa_K1690003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1690003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anna Sosnova, Pavel Zach

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-17 05:34:23

Mating pheromone (L. elongisporus)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1690003_sequence (Version 1)

Description

This is a coding sequence of L. elongisporus mating pheromone together with a secretion tag. Expression in the yeast cell causes secretion of the mature mating pheromone into the medium. Cells with a corresponding STE2 receptor react to this pheromone by the activation of the pheromone mating pathway. Detailed description can be obtained from the iGEM 2015 Czech Republic wiki.

Notes

The secretion tag was obtained by a genomic PCR from S. cerevisiae. The pheromone sequence was synthetized.

Source

This part is derived from BBa_K1690000. Using site-directed mutagenesis, we replaced the original pheromone sequence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Kozak	1,6	ribosome_entry_site, feature/rbs
start	7,9	start_codon, feature/start
Secretion tag	10,275	feature/tag, tag
Pheromone	276,312	sequence_feature, feature/dna
stop	313,315	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1690003/1

Member of these Collections

• iGEM 2018 Distribution Plate 7
• iGEM 2018 Distribution Plate 7 Well 3P
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry