Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Charles Calvet
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-14 12:29:47
cotZ-aeBlue-CIPA
| Types | DnaRegion |
| Roles | Protein_Domain polypeptide_domain |
| Sequences | BBa_K1692028_sequence (Version 1) |
Description
The goal of our project BioHYDRA was to replace all the parts of HYDRAs by biologically produced substances. We sought out to replace polyamide tape by bacterially cellulose, and the glue by cellulose binding domains on the surface of the spore coat. Thus, the first step involved cloning a Bacillus construct in Escherichia coli of a fusion protein sequencing consisting of a spore coat protein, cotZ (building off work done on Sporobeads by the LMU Munich 2012 iGEM team), and a cellulose binding domain (CIPA). Additionally, we decided to add aeBlue, a chromogenic protein, between cotZ and CIPA to be able to see with the naked eye whether Bacillus is in a vegetative or a spore state.Notes
This part was ligated into the pSBbs1c bacillus backbone from the LMU Munich 2012 iGEM team's Bacillus Bio Brick Box (http://parts.igem.org/wiki/index.php?title=Part:BBa_K823023)Source
cotZ from the LMU munich 2012 iGEM team (http://parts.igem.org/Part:BBa_K823032). aeBlue from iGEM12 Uppsala (http://parts.igem.org/Part:BBa_K864401), CIPA from our lab| Access | Instance | Definition |
| public public public | BBa_K1692025 BBa_K1692026 BBa_K1692027 | BBa_K1692025 BBa_K1692026 BBa_K1692027 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1692025 BBa_K1692026 BBa_K1692027 | 1,486 493,1206 1215,1733 | BBa_K1692025 BBa_K1692026 BBa_K1692027 |