BBa_K1698001

BBa_K1698001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1698001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Leanne O Sullivan

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-22 03:41:26

BaseHunter : Human Papiloma Virus (HPV) Detector Part - Can be activated to become detector for 55bp

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1698001_sequence (Version 1)

Description

Part may be activated by established protocol to become a detector plasmid for a 55bp Target sequence, found on the HPV L1 Capsid gene.

For protocol for construction of detector by digestion of plasmid see HERE

The Target is located in the HPV genome flanked by HaeIII restriction sites. Detector may work best if genomic sample first digested with this enzyme to create target fragment of the correct length.

The part was cloned into a plasmid containing BBa_K584001 to allow examination of cells following successful transformation using a fluorescent microscope. Also, presence of GFP expressing part meant that detector colonies could be differentiated from control colonies. For more information see (http://2015.igem.org/Team:Cork_Ireland/Project#standard)

The sequence of this part with K584001 biobrick part may be found on the Registry of Standard Parts

Notes

Target sequence is ideally chosen between two restriction sites to allow digestion of a large genomic sample to yield target fragment of correct size. Also, the size of the target was chosen as it works efficiently with detector.

Source

Target sequence comes from HPV-16 Genome : NC_001526

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1698001/1

Member of these Collections

• iGEM Parts Registry