BBa_K1698002

BBa_K1698002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1698002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Leanne O Sullivan

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-19 09:42:54

BaseHunter : Activated to become detector for 62bp target sequence on SRY gene

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1698002_sequence (Version 1)

Description

Part may be activated by established protocol to become a detector plasmid for a 62bp Target sequence, found on the SRY gene, located on the Y chromosome.

Further optimisation of part is needed for use in distinguishing male and female genomic DNA. Part is accurate in detecting target when isolated from a genomic sample.

For protocol for construction of detector by digestion of plasmid see Wiki

Notes

Target sequence is flanked by HaeIII restriction sites. Digestion of a genomic sample with HaeIII yields fragments of the target size.

Source

62bp Target is from SRY Gene. Accession code : NM_003140

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1698002/1

Member of these Collections

• iGEM Parts Registry