Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Meixi Liu
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 09:56:37
toehold T3 RNAP
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1700001_sequence (Version 1) |
Description
The structure of toehold switch is similar to hairpin, except it has a loop at the top as ???toehold???. Toehold switch functions as riboregulator through linear-linear interaction between RNAs. When target RNA appears, it will bind one of the toehold switch stems and open the loop, exposing the RBS.Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. The switch RNA contains the coding sequence of the gene being regulated. Upstream of this coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon that is followed by a common 21 nt linker sequence coding for low-molecular-weight amino acids added to the N terminus of the gene of interest. A single-stranded toehold sequence at the 50 end of the hairpin module provides the initial binding site for the trigger RNA strand. This trigger molecule contains an extended single-stranded region that completes a branch migration process with the hairpin to expose the RBS and start codon, thereby initiating translation of the gene of interest.
Our toehold is upstream of T3 RNAP. The trigger RNA is miRNA-144.
Notes
The toehold loop can be opened by trigger RNA, thus controlling the expression of T3 RNAP.Source
We sythesis the gene.| Sequence Annotation | Location | Component / Role(s) |
| toehold ATG start codon T3 RNAP TAA stop codon | 1,124 150,152 150,2776 2774,2776 | sequence_feature feature/misc feature/start start_codon CDS feature/cds feature/stop stop_codon |