BBa_K1702000

BBa_K1702000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1702000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anthony Roulier, Chauncy Hinshaw, Krista Henderson

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-17 02:51:24

Overexpression of E. coli fadD under control of lac promoter

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1702000_sequence (Version 1)

Description

Acyl-CoA synthetase is the first enzyme the beta oxidation pathway. It attaches Coenzyme-A to long chain fatty acids (LCFAs), allowing them to continue through the pathway. The transcription of fadD is controlled by the enzyme produced by fadR under standard conditions. In this plasmid, fadD is under control of the lac promoter.

Notes

This plasmid is designed to allow for induced transcription with lactose or IPTG.

Source

This fadD is endogenous to E. coli and was extracted by genomic PCR from DH5alpha E. coli cells.

Components

Access	Instance	Definition
public	BBa_K861010	BBa_K861010
public	BBa_J04500	BBa_J04500

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K861010	1,1605	BBa_K861010
BBa_J04500	1614,1833	BBa_J04500

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1702000/1

Member of these Collections

• iGEM Parts Registry