Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anthony Roulier, Chauncy Hinshaw, Krista Henderson
Date created: 2015-09-16 11:00:00
Date modified: 2016-01-25 01:10:40
E. coli fadD and fadL under control of lac promoter
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1702002_sequence (Version 1) |
Description
Details about fadD:Acyl-CoA synthetase is the first enzyme the beta oxidation pathway. It attaches Coenzyme A to long chain fatty acids, allowing them to continue through the pathway. The transcription of fadD is controlled by the enzyme produced by fadR under standard conditions.
Details about fadL:
LCFAs are naturally moved into E. coli by a long-chain fatty acid outer membrane porin, which is encoded for by fadL.
Detail about P_lac+fadD+fadL construct
By placing both fadD and fadL under the control of an inducible promoter, we can control expression. The combination of fadD and fadL should greatly increase E. coli's uptake of fatty acids into the cell.
Notes
The expression of fadD and fadL is inducible with lactose or IPTG.Source
fadL and fadD were extracted using genomic PCR from DH5alpha E. coli. The lac promoter was sourced from the iGEM distribution kit.| Access | Instance | Definition |
| public public public | BBa_J04500 BBa_K861010 BBa_K861500 | BBa_J04500 BBa_K861010 BBa_K861500 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_K861010 BBa_K861500 | 1,220 227,1831 1838,3178 | BBa_J04500 BBa_K861010 BBa_K861500 |