| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1702004_sequence (Version 1)
|
Description
Synthesized tzs gene (from Agrobacterium tumefaciens C58) and LOG (Oryza satifa), both codon optimized for E. coli as a biobrick. The tzs gene encodes for the enzyme which converts 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate into zeatin riboside 5'-phosphate. The LOG gene produces N6-dimethylallyladenine by removing the phosphoribosyl group from N6-(delta2-isopentenyl)-adenosine 5'-monophosphate. Hydroxylation of the N6-dimethylallyladenine produces biologically-active trans-zeatin.
Notes
The piece was synthesized with the two genes functioning as an operon.
Source
The genes were synthesized based on sequences based on Kamada-Nobusada, Tomoe and Sakakibara, Hitoshi. "Molecular basis for cytokinin biosynthesis." Phytochemistry 70:444-449. March 2009. and found on KEGG (kegg.jp).
