Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sylvie Lautru, Audrey Moatti
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-19 04:53:35
cI857 under the control of RNA thermometer
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1707013_sequence (Version 1) |
Description
The translation of the thermosensitive cI repressor (cI857) is placed under the control of an RNA thermometer. This construction allows the controlled repression of the expression of a gene A with a cI promoter within the 30-35??C range of temperature. Below 30??C, the secondary structure formed by the RNA thermometer in the mRNA prevents the translation of cI857, A is therefore expressed. Above 30??C, the RNA thermometer is unfolded, allowing the translation of cI857. Under 35??, this later is properly folded and active, repressing the expression of A. Above 35??C, cI857 is under an inactive form and A is expressed.Notes
During the design, we had to make sure that the RBS included in the RNA thermometer would be at a correct location in front of the cI857 gene when we will assemble the parts. Luckily, the RNA thermometer part (K115017) had been designed to allow the assembly with protein coding part.Source
This part is the result of the assembly of existing parts.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23101 BBa_K115017 SD cIts BBa_K098997 BBa_B0015 | 1,35 36,118 112,117 119,832 119,832 841,969 | engineered_region feature/BioBrick engineered_region feature/BioBrick ribosome_entry_site feature/rbs CDS feature/cds engineered_region feature/BioBrick engineered_region feature/BioBrick |