Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Astrid Deryckere
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-18 04:12:52
LuxI-His
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1709000_sequence (Version 1) |
Description
Autoinducer synthetase for acyl-homoserine lactones (AHL):LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate.
LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right lux promotor only gives weak constitutive expression of downstream genes.
The LuxI gene is fused to a His-tag facilitating gene expression measurements.
Notes
Biobrick BBa_C0061 still contained a Barcode sequence which was deleted.For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding.
Source
Sequence is originating from V. fischeri.Here, the sequence from BBa_C0061 was used and optimized.
| Sequence Annotation | Location | Component / Role(s) |
| ATG Start Codon LuxI Coding Region Polyhistidine-tag Ochre Stop Codon | 1,3 1,579 583,606 607,609 | start_codon feature/start feature/cds CDS tag feature/tag stop_codon feature/stop |