Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Astrid Deryckere
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 11:21:02
LuxR-E
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1709004_sequence (Version 1) |
Description
Encodes a transcription activator for the right handed Lux promotor.Two molecules of acyl-homoserine lactones (AHL) form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri.
Notes
Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. For our own use, 9 bp were exchanged to create unique restriction sites and to optimize DNA folding.Source
Sequence is originating from V. fischeri. Here, the sequence from BBa_I0462 was used and optimized.| Sequence Annotation | Location | Component / Role(s) |
| ATG Start Codon Ochre Stop Codon E-tag LuxR | 1,3 799,801 753,801 1,750 | feature/start start_codon feature/stop stop_codon feature/tag tag feature/cds CDS |