BBa_K1711000

BBa_K1711000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1711000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anastasia Nicolov

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-18 05:34:22

AFB2 auxin-binding F-box protein

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1711000_sequence (Version 1)

Description

This part, called AFB2, is an F-box protein that binds the common plant hormone auxin. F-box proteins contain an F-box domain that mediates degradation of other proteins by recognizing substrates for ubiquitination.

In our system, a dCas9-degron-repressor complex represses expression of lacZ. In the presence of auxin, the F-box protein and the presence of auxin, the AFB2 binds to auxin and the degron; the F-box protein then recruits the ubiquitin ligase to degrade the dCas9-degron-repressor complex. When this occurs, lacZ is no longer repressed and production of beta galactosidase occurs. Beta galactosidase acts as a reporter for the presence of auxin.

Notes

The parts sequence had to be checked for illegal restriction sites.

Source

AFB2 was originally found in the genome of the species Arabidopsis thaliana, a small flowering plant.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1711000/1

Member of these Collections

• iGEM Parts Registry