Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anastasia Nicolov, Tom Duan, Justin Jenkins
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-19 03:47:39
yeVenus-TheoA
coding sequence for enhanced yellow fluorescent protein and theophylline-sensative ap
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1711001_sequence (Version 1) |
Description
The part contains the coding sequence for yeast enhanced yellow fluorescent protein (yeVenus), linked to it is a gene for a theophylline sensitive riboswitch aptazyme. The aptazyme portion of the transcript self-cleaves in the absence of theophylline and no YFP should be produced. The theophylline bound state stabilizes the transcript, which translates to the protein and fluorescence should be observed.Notes
We had to choose a linker sequence between the Venus and the aptazyme that would be free of secondary structure as a RNA transcript.Source
yeVenus is a modified yellow fluorescent protein, originating from the genome of A. victoria. The theophylline-repsonsive aptamer sequence originates from the genome of S. cerevisiae. Coding sequence for yellow fluorescence protein was given to us by Klavins lab at University of Washington. The aptazyme sequnce was given to us by the Smolke lab at Stanford.[1][1]Townshend B, Kennedy AB, Xiang JS, Smolke CD. High-throughput cellular RNA device engineering. Nature Methods, Jun 2015. doi:10.1038/nmeth.3486
| Sequence Annotation | Location | Component / Role(s) |
| yeVenus 6xGSB Linker Theophylline Aptamer AAAGA | 1,720 721,756 757,860 | ribosome_entry_site feature/rbs sequence_feature feature/misc feature/misc sequence_feature |