Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Our Lady of the Snows Catholic Academy 2015
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-18 11:16:51
Keratinase A under control of LacI promoter.
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1717000_sequence (Version 1) |
Description
This part is a composite of an IPTG-inducible promoter with RBS (BBa_J04500) and a custom-synthesized KERATINASE A expressing plasmid. The coding sequence for Keratinase A was originally isolated in Bacillus, we have now optimized for expression in E.coli. To do this, the signal peptide has been changed.Notes
The bacteria that we are using is a strand of e-coli called K- 12. This strain has a negative granam making it opposite to the gram-positive bacter (Bacillus Licheniformis) the Keratinase that we chose to utilize for our project was found in. After extensive research and looking at the projects of chicago, sheffield and Taiga we learnt that the different ganaam is what causes the issues with the secretion of the keratinase as it gets stuck in the periplasm due to the fact that the signal peptides of the keratin can???t be read by the E. coli. After considering all of this we learned that we must completely remove the gram positive signal sequence, in order to fix this problem. The gram positive signal sequence was completely removed and in its place is PelB (BBa_J32015).Source
Found on the following website: http://www.uniprot.org/uniprot/Q53521.| Access | Instance | Definition |
| public public | BBa_J04500 BBa_K1717171 | BBa_J04500 KERA |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_K1717171 | 1,220 229,1341 | BBa_J04500 KERA |