BBa_K1718001

BBa_K1718001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1718001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Naomi Iverson
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-13 06:37:15

pBAD promoter with RBS-RFP



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K1718001_sequence (Version 1)

Description

To test the leakiness of the pBAD promoter, a plasmid must be assembled with a gene that indicates when the promoter is stimulated by L-arabinose. To do this, we chose Red Fluorescent Protein (RFP) to help signal when the promoter responds.

Notes

We removed the RFP promoter (from BBa_J04450) so that RFP would only be transcribed when pBAD was stimulated by arabinose.

Source

All parts from this construct come from the registry.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1718001/1