Types | DnaRegion
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Roles | engineered_region
Reporter
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Sequences | BBa_K1718001_sequence (Version 1)
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Description
To test the leakiness of the pBAD promoter, a plasmid must be assembled with a gene that indicates when the promoter is stimulated by L-arabinose. To do this, we chose Red Fluorescent Protein (RFP) to help signal when the promoter responds.
Notes
We removed the RFP promoter (from BBa_J04450) so that RFP would only be transcribed when pBAD was stimulated by arabinose.
Source
All parts from this construct come from the registry.