BBa_K1718002

BBa_K1718002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1718002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daren Kraft and Josephina Hendrix

Date created: 2015-09-12 11:00:00

Date modified: 2015-09-15 02:08:17

Genetic Switch for Bxb1 invertase

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K1718002_sequence (Version 1)

Description

This part consists of a strong Anderson promoter and a terminator flanked by attB and attP, the recognition sites for BxbI. In its off position, the terminator prevents expression of the downstream part. If the BxbI invertase is expressed, it will recognize the attB and attP sites and invert the DNA; thus, flipping the terminator so polymerase can pass through the gate.
The Bxb1 invertase flips DNA using the attB and attP sites. There is a Bxb1 excisionase that can flip the DNA back.

Notes

The design consists of a terminator to stop transcription from the strong constitutive promoter.

Source

Sequences are based on the Endy experiments.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1718002/1

Member of these Collections

• iGEM Parts Registry