BBa_K1718003

BBa_K1718003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1718003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hannah Young

Date created: 2015-09-13 11:00:00

Date modified: 2015-09-14 03:23:55

N-acyl-homoserine-lactone inducible LuxPr promoter generating RFP

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1718003_sequence (Version 1)

Description

The part consists of LuxR followed by the AHL inducible LuxPr promoter which is placed in front of RFP.
This part contains BBa_F2620 which is composed of a constitutive TetR promoter which produces the LuxR transcription factor. Followed by that is the LuxPr promoter, which is induced when a N-acyl-homoserine-lactone molecule binds to the LuxR transcription factor. Following this promoter is the reporter RFP.

Notes

We removed the promoter from BBa_J04450. The TetR promoter and LuxR gene are necessary for induction of the promoter.

Source

The LuxR part is BBa_F2620 from the 2015 iGEM Distribution Kit. RFP or BBa_J04450 was also obtained from the 2015 iGEM Distribution Kit.

Components

Access	Instance	Definition
public	BBa_F2620	BBa_F2620
public	BBa_B0034	BBa_B0034
public	BBa_E1010	mRFP1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_F2620	1,1061	BBa_F2620
BBa_B0034	1070,1081	BBa_B0034
BBa_E1010	1088,1793	mRFP1

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1718003/1

Member of these Collections

• iGEM Parts Registry