Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Donovan
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-18 07:12:07
Genetic Switch LuxI GFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1718006_sequence (Version 1) |
Description
This part consists of a switch composed of a strong Anderson promoter and a terminator flanked by attB and attP, the recognition sites for BxbI. In its off position, the terminator prevents expression of the downstream part. If the BxbI invertase is expressed, it will recognize the attB and attP sites and invert the DNA; thus, flipping the terminator so polymerase can pass through the gate. The Bxb1 invertase flips DNA using the attB and attP sites.After the switch the are sequences that code for luxI, the autoinducer synthetase for AHL, and GFP.
Notes
Composite part using a genetic switch, part of the lux cassette of V. fischeri, and GFP.Source
Gate from IDT with LuxI and GFP| Access | Instance | Definition |
| public public | BBa_K1718002 BBa_K1718004 | BBa_K1718002 BBa_K1718004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1718002 BBa_K1718004 | 1,293 302,1851 | BBa_K1718002 BBa_K1718004 |