BBa_K1720002

BBa_K1720002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1720002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yuanbin Cui, Yaran Zhao

Date created: 2015-08-24 11:00:00

Date modified: 2015-09-18 07:26:50

Hypoxia-induced promotor

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1720002_sequence (Version 1)

Description

This is a part used for coding soluble human guanylate cyclase subunit:Alpha 3.Alpha 3 unit interacts with a beta subunit to form the guanylate cyclase enzyme.
Soluble guanylate cyclases(sGC) are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. It is important for smooth muscle relaxation in the cardiovascular system. sGC contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs.There are several different isoforms of sGC subunit ,but the cardiovascular system abundant with alpha 3 unit and beta 3 unit.

This part with a GFP reporter was then transfected into HEK293 cells by lentiviral Vector and in vivo green fluorescence signal was observed under fluorescence microscope, it meant that this part was transfected into HEK293 cells successfully.Then we used qRT-PCR to observe transcriptional level of alpha 3 unit.Finally we detected the cGMP concentration with Elisa Kit. And the results are as follow:

Notes

This part do not have promoter because the promoter we used in our project is EF1A which consists the restriction sites.So before using this part please add a promoter.

Source

We find the information of Human guanylate cyclase??1,soluble, alpha 3 unit here：http://www.ncbi.nlm.nih.gov/gene/2982

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1720002/1

Member of these Collections

• //biosafety
• //regulation
• iGEM Parts Registry