aa-tRNA

BBa_K1722004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1722004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yongyi Wang

Date created: 2015-08-25 11:00:00

Date modified: 2015-09-07 08:29:17

This is a kind of aminoacyl-tRNA(aa-tRNA).

Details

• Types: DnaRegion
• Roles: RNA; mature_transcript_region
• Sequences: BBa_K1722004_sequence (Version 1)

Description

This is a kind of aminoacyl-tRNA, which has a diffeent structure compared with classic tRNA.The tRNA can only carry a specific amino acid, and is non natural amino acids.It has special anticodons(CUA),which can identify the termination codon(UAG), and then adds the unnatural amino acid carried own into the polypeptide chain to.So that the position of the termination codon corresponds to the insertion of an unnatural amino acid, which allows the protein to continue to be translated.
The aminoacyl-tRNA came from barchaebacteria,and it is generally applied to unnatural amino acid orthogonal system.
In our project, the aminoacyl-tRNA was selected by directed evolution.

Notes

Because itonly has 72 bases, and the traditional PCR method is not applicable for this,we get his by gene synthesising.

Source

It came from barchaebacteria.

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1722004/1

Member of these Collections

• iGEM Parts Registry