Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Emilie Cuillery
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-08 05:58:31
PAM rich URS j23117 promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1723001_sequence (Version 1) |
Description
PAM rich URS J23117 is a promoter made from the existing J23117 promoter. This promoter has PAM rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004).All this system is operating in the special cell strain JEN202. In our experiments this promoter is 27 base pairs upstream the ATG start codon, an average of 30bp is recommended.
Notes
PAM rich sequence was a bit shortened comparing to the original promoter in pWJ89 plasmid as it contained one RFC10 restriction site but all the binding sites remains intact.Source
This promoter was shipped to us by Dr David Bikard, from the pasteur institute, in the pWJ89 plasmid.| Sequence Annotation | Location | Component / Role(s) |
| PAM sgRNA Z4 recognition site PAM sgRNA Z35 recognition site PAM sgRNA Z0 recognition site J23117 | 13,15 16,35 44,46 47,66 71,73 51,70 50,84 | feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature promoter feature/promoter |