Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Emilie Cuillery
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-15 12:15:33
sgRNA X35 expressing cassette
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K1723008_sequence (Version 1) |
Description
This part is expressing the sgRNA (single guide RNA) X35 that can bind to dCas9-ω (BBa_K1723000). The complex was designed to bind the PAM rich URS J23117Alt promoter (BBa_K1723005) to repress the production of the target gene linked to the promoter. This part is a part of a gene regulation system built with dCas9-ω, with the parts sgRNA X0 expressing cassette (BBa_K1723006) and gRNA X35 expressing cassette (BBa_K1723008). This sgRNA coding sequence was biobricked with the promoter pBAD and its terminator, both used for our experiments. As the gRNA sequence has to start the first base after the promoter it couldn't have been used with the standard biobrick system so the promoter is provided already. It is possible to PCR out the part to put it under another promoter, but he terminator is specific and it is recommended to keep it.Discover all the parts that can work with this one:
http://2015.igem.org/Team:EPF_Lausanne/Part_Collection
| Sequence Annotation | Location | Component / Role(s) |
| pBAD promoter X35 specific sequence sgRNA scaffold S. Pyogenes terminator | 1,173 174,193 194,235 236,276 | feature/promoter promoter sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature |