Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cyril Pulver
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 01:36:09
CYC_0 promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1723022_sequence (Version 1) |
Description
CYC_0 is the pCYC Yeast Promoter (BBa_K124000) preceded by a PAM sequence (NGG) rich region that allows binding of dCas9_VP64 together with the appropriate guide RNA (gRNA) to 20 base pair specificity determinant sequences flanked by PAM sequences. It is activated by binding of the complex formed of dCas9_VP64 (BBa_K1723021) and c3_0 gRNA (produced by BBa_K1723009): recruitment of RNA polymerase II by the VP64 subunit on the promoter will express a downstream gene. It is inhibited by binding of the complex formed of dCas9_VP64 and c6_0 gRNA (produced by BBa_K1723013): the complex will sterically prevent RNA polymerase II from binding to the promoter. It is inhibited through the same mechanism by binding of the complex formed of dCas9_VP64 and c7_0 gRNA (produced by BBa_K1723017). If any combination of those complexes is expressed, the promoter will be inhibited [1].To learn more about how this part was used specific to our project, please follow this link: http://2015.igem.org/Team:EPF_Lausanne/Part_Collection
Notes
This promoter was specifically designed to be controlled by complexes constituted of dCas9_VP64 and gRNAs c3_0, c6_0 and c7_0. Since it has been demonstrated that inhibition prevails over activation when combining the co-expression of dCas9_VP64 and those gRNAs [1], this promoter can be used as a basic cellular computation tool.Source
Synthesized as a G-Block| Sequence Annotation | Location | Component / Role(s) |
| c3_0 activation site c7_0 inhibition site c6_0 inhibition site | 39,58 58,77 126,145 | non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site |