| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_K1725000_sequence (Version 1)
|
Description
The promoter is on normally, and off when the PhlF repressor (K1725041) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725020.
Notes
We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html They based the promoter sequence on J23119, then overlapped the operator sequence of the PhlF repressor over the -10 site.
Source
From "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
