BBa_K1725001

BBa_K1725001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1725001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mhairi Davidson
Date created: 2015-08-10 11:00:00
Date modified: 2016-07-23 11:17:34

PhlF repressible promoter + strong RBS + GFP



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K1725001_sequence (Version 1)

Description

The promoter is on normally, and off when the PhlF repressor (K1725041) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725020.

Notes

.

Source

PhlF promoter from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html

Access Instance Definition
public
public
BBa_K1725000
BBa_I13500
P-phlF
BBa_I13500
igem#experience
Works
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1725001/1