Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mhairi Davidson
Date created: 2015-08-06 11:00:00
Date modified: 2015-09-18 03:58:50
SrpR repressible promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1725020_sequence (Version 1) |
Description
The promoter is on normally, and off when the SrpR repressor (K1725061) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725000.Notes
We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html They based the promoter sequence on J23119, then overlapped the operator sequence of the SrpR repressor over the -10 site.Source
From "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html| Sequence Annotation | Location | Component / Role(s) |
| K1725060 operator sequence | 38,67 | feature/operator operator |