BBa_K1725021

BBa_K1725021 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1725021

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mhairi Davidson

Date created: 2015-08-10 11:00:00

Date modified: 2015-08-11 09:32:10

SrpR repressible promoter + RBS + GFP

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1725021_sequence (Version 1)

Description

The promoter is on normally, and off when the SrpR repressor (K1725061) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725000.

Notes

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Source

SrpR promoter rom "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html

Components

Access	Instance	Definition
public	BBa_K1725020	P-srpR
public	BBa_I13500	BBa_I13500

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1725020	1,67	P-srpR
BBa_I13500	76,813	BBa_I13500

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1725021/1

Member of these Collections

• iGEM Parts Registry