BBa_K1725022

BBa_K1725022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1725022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mhairi Davidson

Date created: 2015-08-10 11:00:00

Date modified: 2015-08-11 09:39:34

SrpR repressible promoter + RBS + GFP

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1725022_sequence (Version 1)

Description

The promoter is on normally, and off when the SrpR repressor (K1725061) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725000.

Notes

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Source

SrpR promoter from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html

Components

Access	Instance	Definition
public	BBa_K1725020	P-srpR
public	BBa_E5501	BBa_E5501

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1725020	1,67	P-srpR
BBa_E5501	76,814	BBa_E5501

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1725022/1

Member of these Collections

• iGEM Parts Registry