Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrey Filipov
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-08 11:23:40
PlsiR + B0034 + GFP
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1725401_sequence (Version 1) |
Description
The promoter activated by the release of UirR (K1725420) from the transmembrane protein UirS (K1725410) in response to unidirectional UV-A light is upstream of GFP with the B0034 RBS (BBa_I13500). This allows activity in response to UV-A to be measured. For the promoter to be activated UirR and UirS must be present and the cell has to be exposed to UV-A.Notes
The promoter, including the BioBrick prefix and suffix, was ordered as a G-block. It was ligated to I13500 after restriction that was followed by an Azure-A-based gel extraction.Source
The promoter comes from the genome of the unicellular cyanobacterium Synechocystis sp. PCC6803.| Access | Instance | Definition |
| public public | BBa_K1725400 BBa_I13500 | PlsiR BBa_I13500 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1725400 BBa_I13500 | 1,534 543,1280 | PlsiR BBa_I13500 |