Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ying Kuan
Date created: 2015-09-01 11:00:00
Date modified: 2016-02-03 10:04:50
Signiferin with signal peptide
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1727000_sequence (Version 1) |
Description
Antimicrobial peptides (AMPs) are stable peptides that have extensive ability to kill or inhibit the growth of bacteria. They play a role in defense mechanism for all organisms, ranging from prokaryotes to humans, to against predators. Some of them act against specific strain of microbes.Signiferin is a kinds of antimicrobial peptide extracted from the skin mucus of Crinia signifera, respectively. It demonstrated effectiveness in killing MRSA. And we had change it's sequence into E.coli codon usage. We treat a signal peptide in front of signiferin. This signal peptide comes from S.lividas and has ability to let E.coli secrect signiferin to LB culture medium.
Notes
We had change signiferin sequence into E.coli codon usage. In order to get the antimicrobial peptide easily we treat signal peptide in front of the AMP.Source
We synthesis this sequence from IDT (Integrated device technology) company.| Sequence Annotation | Location | Component / Role(s) |
| Restriction enzyme site Nco I signal peptide Signiferin Restriction enzyme site BamH I | 1,6 7,104 105,173 173,179 | feature/misc sequence_feature sequence_feature feature/dna feature/protein CDS feature/misc sequence_feature |