Trg-EnvZ

BBa_K1731000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1731000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alejandro Rodriguez-Gama, Lizbeth Airais Bola??os Castro

Date created: 2015-09-13 11:00:00

Date modified: 2015-09-16 02:00:58

Constitutive Trg-EnvZ Chimera

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1731000_sequence (Version 1)

Description

The Chimera Trg-EnvZ is a two component system that constitutes a chimeric protein composed of the extracellular and transmembrane domains of chemoreceptor Trg and the cytosolic kinase domain of osmosensor EnvZ . Trg is activated by periplasmic binding proteins, and transduces the message to EnvZ to phosphorylate OmpR, a transcription factor which binds to the OmpC promoter driving transcription of whatever gene that is downstream.

Notes

Some restriction sites were removed without altering the open reading frame.

Source

Synthetic and codon optimized for E. coli expression

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23101	1,35	feature/promoter, promoter
BBa_B0034	45,56	ribosome_entry_site, feature/rbs
TrgZ	63,1550	feature/cds, CDS
BBa_B0015	1560,1688	feature/BioBrick, engineered_region

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1731000/1

Member of these Collections

• iGEM Parts Registry