Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Donna Lee
Date created: 2015-09-02 11:00:00
Date modified: 2015-09-18 09:27:02
J23100-CDcel-GlucCO-B0015
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K1732001_sequence (Version 1) |
Description
J23100 Promoter with a RBS (BBa_S04423), CDcel domain, Gaussia princeps luciferase, and a B0015 terminator. Allows Gaussia luciferase to be constitutively transcribed and translated. Luminescence can be expressed by reacting the luciferase product with coelenterazine.Notes
The sequence is codon optimized for E.coli.The plasmid was used to produce Gaussia princeps luciferase proteins that would express luminescence when reacted with an optimal concentration of 1 uM coelenterazine. The CDcel domain allowed for the proteins to be secreted into the media (extracellular) which was both the expected and observed location.
Source
Provided by the Bruchez lab at Carnegie Mellon University.| Sequence Annotation | Location | Component / Role(s) |
| J23100 RBS J23100 RBS CDcel Gaussia princeps luciferase 6XHIS B0015 terminator | 2,36 43,54 154,1188 1204,1707 1708,1725 1738,1866 | feature/promoter promoter promoter feature/promoter feature/cds CDS CDS feature/protein feature/tag tag feature/misc sequence_feature |