BBa_K1734006

BBa_K1734006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1734006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eduardo Ram??rez Montiel

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 08:29:09

6xHis-T2A-Nanoluc

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1734006_sequence (Version 1)

Description

This part encodes a sequence capable to add a purification tag (6X His) at the 3??? terminus of a desired protein. Furthermore, by using the reporter gene (nanoluc) it???s possible to quantify indirectly the protein concentration. The T2A sequence cleaves the protein [1], producing the protein of interest tagged with HisTag and nanoluc separately
This part has been codon optimized for Spodoptera frugiperda (Sf9)

Reference:
[1] Kim JH, Lee S-R, Li L-H, Park H-J, Park J-H, Lee KY, et al. (2011) High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice. PLoS ONE 6(4): e18556. doi:10.1371/journal.pone.0018556

Notes

The sequence produces two proteins separately, the protein of interest and nanoluc

Source

Genetic design

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
6xHis Tag	1,18	tag, feature/tag
T2A sequence	19,74	feature/misc, sequence_feature
Nanoluc	75,599	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1734006/1

Member of these Collections

• iGEM Parts Registry