BBa_K174000

BBa_K174000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K174000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: The Newcastle 2009 iGEM team

Date created: 2009-09-25 11:00:00

Date modified: 2015-05-08 01:10:58

SspB proteolysis chaperone

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K174000_sequence (Version 1)

Description

SspB protein targets proteins tagged with modified degradation tag, ssrA, and deliver them to ClpXP for proteolysis. Modified ssrA tags are fused onto the 3??? end of a gene.

Notes

Forward primer used: GATCTG-GAATTCGCGGCCGCTTCTAG-ATGGATTTGTCACAGCTAAC
(Clamp sequence - Standard Biobrick prefix - first 20 base from the Biobrick)
Reverse primer used: TGTGAC-ACTAGTA-TTACTTCACAACGCGTAATGC
(Clamp sequence - SpeI site - last 21 base from the Biobrick)

Source

498 bp long sspB CDS is amplified by PCR with dangling end primers with EcoRI-NotI-XbaI restriction site at 5??? and SpeI at 3??? and inserted into a Biobrick compatible vector. The sequence is taken from E. coli strain DH5 alpha. Genbank accession number for E. coli MG1655 strain is NC_000913.2

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
sspB	1,498	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K174000/1

Member of these Collections

• //cds
• //function/degradation
• iGEM Parts Registry