Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: The Newcastle 2009 iGEM team
Date created: 2009-09-25 11:00:00
Date modified: 2015-05-08 01:10:58
Arabinose inducible system
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K174001_sequence (Version 1) |
Description
Comprised of araR binding sites and araE promoter. Regulates the expression of downstream genes with arabinose.Notes
Forward primer used: GATCTG-GAATTCGCGGCCGCTTCTAGAG-CACAGCGTTCTTTGTAAG(Clamp sequence - Standard Biobrick prefix - first 18 base from the Biobrick)
Reverse primer used: TGTGAC-ACTAGTA-GCCCTCCCGAATGTTGAG
(Clamp sequence - SpeI site - last 18 base from the Biobrick)
Source
Wild-type Bacillus subtilis 168 strain. 200 bp long bindingsite_promoter_bindingsite_RBS is amplified by PCR with dangling end primers with EcoRI-NotI-XbaI restriction site at 5??? and SpeI at 3??? and inserted into a Biobrick compatible vector. The sequence is taken from wildtype Bacillus subtilis| Sequence Annotation | Location | Component / Role(s) |
| araR binding site sigA promoter araR binding site rbs | 32,51 80,123 154,178 208,214 | non_covalent_binding_site feature/binding feature/promoter promoter non_covalent_binding_site feature/binding feature/rbs ribosome_entry_site |