Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: The Newcastle 2009 iGEM team
Date created: 2009-10-09 11:00:00
Date modified: 2015-05-08 01:10:58
xylA promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K174005_sequence (Version 1) |
Description
This promoter can be induced by xylose and it is regulated by LacI. XylR is normally expressed i B. subtilis and represses the promoter. Addition of xylose will derepress XlyR activity on the promoter.Biobrick BBa_K143014 is also xylose inducable promoter. They have slightly different operator sites and -10 sequence is one base different. Hence this promoter may have different promoter strength and regulation by XlyR.
It should be noted that glucose may act as an anti-inducer for xlyR triggering catabolite repression.
Notes
The sequence from the beginning of xlyA promoter to the start codon just before SpeI site was used to build the part.Source
This part is based on pX integration vector which has xlyA promoter with xlyR operator sites.| Sequence Annotation | Location | Component / Role(s) |
| -10 -35 XylR operator XylR Operator | 28,33 5,10 43,52 58,67 | feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding feature/operator operator feature/operator operator |