Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 04:48:14
sfGFP under arabinose promoter s1 without AraC (Arashort1)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1741000_sequence (Version 1) |
Description
In commercially available expression vectors arabinose promoter araBAD is usually used together with a gene for AraC transcription activator / repressor to make the system independent of chromosomal copy of AraC gene. We fused the araBAD promoter without AraC reading frame, as other sugar induced promoters to sfGFP. The shorter version of the popular promoter is still functional i.e. induced by arabinose.Notes
We fused the araBAD promoter without AraC reading frame, as other sugar induced promoters to sfGFP.Source
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| O2 region O1 region CAP binding site I1, I2 binding site -35 signal -10 signal 5'UTR His-tag two consecutive STOP codons sfGFP | 1,16 158,179 203,213 215,247 245,249 269,273 283,309 310,339 1054,1059 340,1053 | feature/operator operator operator feature/operator non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature tag feature/tag feature/stop stop_codon feature/protein CDS |