Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 07:28:47
sfGFP under promoter xylA1 with improved 5'UTR
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1741008_sequence (Version 1) |
Description
Xylose induced promoter xylA is weaker than arabinose and rhamnose promoters. To enhance the efficiency of xylA driven transcription/translation we have transplanted 5???UTR from the strong constitutive promoter proD. In some experiments it seems to be slightly stronger than original xylA promoter from part BBa_K1741007.Notes
We have transplanted 5'UTR from the strong constitutive promoter proD.Source
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| CRP binding site XylR binding site XylR binding site -35 signal -10 signal modified 5'UTR part of 5'UTR taken from proD promoter His-tag sfGFP two consecutive STOP codons | 235,253 254,268 274,289 290,294 313,318 324,376 338,376 377,406 407,1120 1121,1126 | non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/tag tag feature/protein CDS feature/stop stop_codon |