Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 06:33:37
sfGFP under xylose promoter xylF (very weak)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1741010_sequence (Version 1) |
Description
We also tested the xylF promoter from the same double sided regulatory element (cf. BBa_K1741007), and xylF appears to be a very weak promoter slightly induced by xylose.Notes
None.Source
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| IF1 IF2 -35 signal -10 signal 5'UTR His-Tag sfGFP two consecutive STOP codons | 43,58 63,78 86,90 107,112 119,180 181,210 211,924 925,930 | non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc tag feature/tag CDS feature/protein feature/stop stop_codon |