Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 06:40:47
sfGFP under T7 promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1741011_sequence (Version 1) |
Description
This part imitates the most popular IPTG/lactose dependent T7 driven expression systems. To express sfGFP or another sequence with which one can substitute sfGFP ORF using Gibson or CPEC assembly, host strain of choice expressing T7 RNA polymerase is necessary, not only DE3.Notes
None.Source
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter T7 terminator operator lac His-tag sfGFP two consecutive STOP codons 5'UTR | 1,20 839,886 21,44 89,118 119,832 833,838 20,88 | promoter feature/promoter sequence_feature feature/misc operator feature/operator tag feature/tag CDS feature/protein feature/stop stop_codon feature/misc sequence_feature |