Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 06:48:58
sfGFP under proC promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1741012_sequence (Version 1) |
Description
proC moderately strong constitutive promoter (cf. Davis 2010) seems to reflect growth rate of E. coli bacterial strains on different media, and potentially can be a measure of protein biosynthesis rate, to be determined in future... Expression is higher than of inducible promotersNotes
None.Source
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| proC promoter -35 signal -10 signal 5'UTR His-tag sfGFP two consecutive STOP codons | 1,101 63,68 86,91 102,158 159,188 189,902 903,908 | feature/promoter promoter feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/tag tag feature/protein CDS stop_codon feature/stop |