Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mansi Chandra
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-18 12:10:38
KaiABC Oscillator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1745001_sequence (Version 1) |
Description
pMC001 exhibits the three Kai proteins: KaiA, KaiB, and KaiC, which constitute a circadian oscillator. This biobrick can be used to drive circadian processes. Oscillations can be tracked through the phosphorylation states of KaiC.Notes
In vitro investigations of this modular system revealed that the robustness of oscillations was sensitive to stoichiometric ratios of KaiA to KaiC (Nakajima, Science 2015). To experiment with these stoichiometric ratios, KaiB and KaiC expression are driven under a constitutive promoter (Part J23100) while KaiA expression is driven under a L-rhamnose inducible promoter (Part K914003).Source
The KaiABC circadian oscillator is endogenous to the cynanobacterial species Synechococcus elongatus.| Sequence Annotation | Location | Component / Role(s) |
| pRha Inducible BBa_K914003 RBS BBa_B0034 Kai A Double Terminator BBa_B0014 Kai C RBS BBa_B0034 Kai B RBS BBa_B0034 Constitutive BBa_J23100 | 1,121 127,138 144,1001 1002,1090 1091,2652 2658,2670 2677,2983 2990,3006 3013,3045 | promoter feature/promoter ribosome_entry_site feature/rbs feature/protein CDS engineered_region feature/BioBrick feature/protein CDS feature/rbs ribosome_entry_site feature/protein CDS feature/rbs ribosome_entry_site promoter feature/promoter |